Example of a complete action potential dataset used for plotting and analyses. This data contains parameters like peak_amplitude, after-hyperpolarization amplitude, and half-width for two time-points: during the control period (state == "Baseline") or after insulin has been applied (state == "Insulin").

This is an example of what the data looks like after being imported with add_new_cells() with data_type = "AP". You can see that the same columns from the cell characteristics file have been appended to new rows for peak_amplitude, after-hyperpolarization amplitude, latency to fire, and others.

Format

An .rda file containing 34 objects of 25 variables.

letter, synapses, ID, sex, animal, treatment, etc.: All columns that were produced in add_new_cells(). Please see sample_cell_characteristics for detailed column descriptions.
state: A character ("Baseline" or "Insulin") representing the timepoint that the data point belongs to.
time_of_threshold: The time (in ms) when the membrane potential reaches the threshold value.
threshold: The threshold (in mV). Determined using the first derivative method, where the threshold is the membrane potential which results in a derivative of 10 mV/ms or greater (Farries et al., 2010).
t_11: The value of the first derivative (action potential velocity in mV/ms) at threshold.
first_sweep_with_APs: The sweep number of the first sweep (going from lowest to higher current injection values) that resulted in an action potential.
trace_start: An automated value in Clampfit that is not used in the analysis.
peak_amplitude: The peak amplitude (in pA) of the action potential relative to threshold.
time_to_peak: The time to peak amplitude (in ms) relative to the time of threshold.
antipeak_amplitude: The after-hyperpolarization amplitude (in pA) relative to threshold.
time_of_antipeak: The time of the after-hyperpolarization (in ms).
half_width: The half-width, which is the width of the action potential and half of the peak amplitude.
latency_to_fire: The latency to fire, which is calculated by subtracting the injection start time from the time_to_peak.
antipeak_time_relative_to_threshold: The time of the afterhyperpolarization amplitude (in ms) relative to the time of the threshold.

Format

See also