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This is an example of the summary eEPSC data produced using make_summary_EPSC_data(). It is useful for demonstrating functions that build off of this dataset, such as plot_summary_current_data().

Format

An .rda file containing 95 objects of 20 variables.

category

A factor representing the experiment type. Used to assign top-level groups for further analyses, with treatment as subgroups.

letter

A factor that is a unique identifier for a single recording. Used to link data sets for evoked or spontaneous currents and cell-characteristics.

sex

A factor with two levels (e.g. "Male" or "Female").

treatment

A factor based on the treatment applied (e.g. "Control", "HNMPA"). This represents the antagonists or agonists applied, or any protocol applied to the animals (e.g. "Fasting").

interval

A factor indicating the interval that the data point belongs to. For example, interval will be "t0to5" for any data points from 0 to 5 minutes. Example values: "t0to5", "t5to10", etc.

mean_P1_transformed

A numeric value representing the mean current amplitude (in pA normalized to the baseline amplitude) of the first evoked current. The values used to produce mean_P1_transformed come from all data points within each interval (the length of the interval was specified in make_normalized_EPSC_data()).

mean_P1_raw

The same values as those in mean_P1_transformed except these values contain raw values for current amplitude (e.g. the data were not baseline transformed).

n

The number of data points used to produce each value in mean_P1_transformed and mean_P1_raw

sd

The standard deviation of the mean current values.

cv

The coefficient of variation of the mean current values.

se

The standard error of the mean current values.

cv_inverse_square

The inverse coefficient of variation squared of the mean current values. Calculated by using (1/cv^2), where cv is the coefficient of variation. Useful for variance analysis.

variance

The variance of the current values, calculated using stats::var().

VMR

The variance-to-mean ratio, calculated using variance/mean_P1_transformed. Useful for variance analysis.

age

A numeric value representing the animal's age. Can be any value as long as the time units are consistent throughout (e.g. don't mix up days and months when reporting animal ages).

animal

A numeric value representing the animal's ID or number.

X

A numeric value representing the x-value of the cell's location in µm. Leave this blank if you don't have this data.

Y

A numeric value representing the y-value of the cell's location in µm. Leave this blank if you don't have this data.

time

A numeric value that represents time in minutes. This column is autogenerated in add_new_cells().

synapses

A character value (e.g. "Glutamate" or "GABA").