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This is an example of the summary eEPSC data produced using make_summary_EPSC_data(). It is useful for demonstrating functions that build off of this dataset, such as plot_summary_current_data().

Format

An .rda file containing 70 objects of 16 variables.

category

A factor representing the experiment type. Used to assign top-level groups for further analyses, with treatment as subgroups. For example, "1" may refer to an experiment where you applied high-frequency stimulation (HFS) to a cell, while "2" is an experiment where you added a hormone like leptin. "3" may be an experiment where you applied HFS in the continuous presence of leptin.

letter

A factor that is a unique identifier for a single recording. Used to link data sets for evoked or spontaneous currents and cell-characteristics.

sex

A factor with two levels (e.g. "Male" or "Female").

treatment

A factor based on the treatment applied (e.g. "Control", "HNMPA"). This represents the antagonists or agonists applied, or any protocol applied to the animals (e.g. "Fasting").

interval

A factor indicating the interval that the data point belongs to. For example, interval will be "t0to5" for any data points from 0 to 5 minutes. Example values: "t0to5", "t5to10", etc.

mean_transformed_amplitude

The average normalized spontaneous current amplitude (% Baseline sEPSC amplitude).

mean_raw_amplitude

The average raw spontaneous current amplitude (pA).

n

The number of datapoints used to create the average.

sd_transformed_amplitude

The standard deviation of the normalized spontaneous current data (mean_transformed_amplitude).

se_transformed_amplitude

The standard error of mean_transformed_amplitude.

mean_transformed_frequency

The average normalized frequency (% Baseline frequency).

sd_transformed_frequency

The standard deviation of mean_transformed_frequency.

se_frequency

The standard error of mean_transformed_frequency.

mean_raw_frequency

The average raw frequency (Hz).

time

A numeric value that represents time in minutes. This column is autogenerated in add_new_cells().

synapses

A character value (e.g. "Glutamate" or "GABA").

days_alone

A numeric value describing the number of days that the animal was left alone in a cage. This typically ranges from 0 to 2. Fasted animals will have 1 day alone.

animal_or_slicing_problems

A character value ("yes" or "no") describing if there were any issues with the animal (for example, the animal was unusually anxious) or slicing (there were delays during the process, the slices were crumpling, etc.).