Spontaneous current data summarized into 5-minute intervals
Source:R/Sample-datasets.R
sample_summary_sEPSC_df.RdThis is an example of the summary eEPSC data produced using
make_summary_EPSC_data(). It is useful for demonstrating functions that
build off of this dataset, such as plot_summary_current_data().
Format
An list of 5 dataframes. The first dataframe ($summary_data) contains spontaneous current data for each cell, averaged per interval. The second and third dataframes ($percent_change_amplitude and $percent_change_frequency) have columns expressing the percent change in spontaneous current amplitude or frequency in the last time interval (t15to20) relative to the baseline (t0to5). The third and fourth dataframes ($mean_SE_amplitude and $mean_SE_frequency contain mean and standard deviation and error values for each interval for spontaneous current amplitude and frequency, respectively.
$summary_data
categoryA factor representing the experiment type. Used to assign top-level groups for further analyses, with
treatmentas subgroups. For example, "1" may refer to an experiment where you applied high-frequency stimulation (HFS) to a cell, while "2" is an experiment where you added a hormone like leptin. "3" may be an experiment where you applied HFS in the continuous presence of leptin.letterA factor that is a unique identifier for a single recording. Used to link data sets for evoked or spontaneous currents and cell-characteristics.
sexA factor with two levels (e.g. "Male" or "Female").
treatmentA factor based on the treatment applied (e.g. "Control", "HNMPA"). This represents the antagonists or agonists applied, or any protocol applied to the animals (e.g. "Fasting").
intervalA factor indicating the interval that the data point belongs to. For example,
intervalwill be "t0to5" for any data points from 0 to 5 minutes. Example values: "t0to5", "t5to10", etc.mean_transformed_amplitudeThe average normalized spontaneous current amplitude (% Baseline sEPSC amplitude).
mean_raw_amplitudeThe average raw spontaneous current amplitude (pA).
nThe number of datapoints used to create the average.
sd_transformed_amplitudeThe standard deviation of the normalized spontaneous current data (
mean_transformed_amplitude).se_transformed_amplitudeThe standard error of
mean_transformed_amplitude.mean_transformed_frequencyThe average normalized frequency (% Baseline frequency).
sd_transformed_frequencyThe standard deviation of
mean_transformed_frequency.se_frequencyThe standard error of
mean_transformed_frequency.mean_raw_frequencyThe average raw frequency (Hz).
timeA numeric value that represents time in minutes. This column is autogenerated in
add_new_cells().synapsesA character value (e.g. "Glutamate" or "GABA").
days_aloneA numeric value describing the number of days that the animal was left alone in a cage. This typically ranges from 0 to 2. Fasted animals will have 1 day alone.
animal_or_slicing_problemsA character value ("yes" or "no") describing if there were any issues with the animal (for example, the animal was unusually anxious) or slicing (there were delays during the process, the slices were crumpling, etc.).
The second and third dataframes contain percent change data for spontaneous current amplitude and frequency, respectively. The columns are the same as the ones produced for evoked currents (read the documentation for sample_summary_eEPSC_df$percent_change_data.
The fourth and fifth dataframes contain the mean, SE, SD, and n data for spontaneous current amplitude and frequency, respectively. Read the description for the sample_summary_eEPSC_df$mean_SE to learn about these columns.